SPECTROLINKER UV CROSSLINKERS
Microprocessor-Controlled UV Crosslinker Makes All the Others Obsolete!
Spectrolinker — the World’s Most Advanced, Versatile and Accurate UV Crosslinker is Also the Easiest to Use!
The Spectrolinker was designed to be one of the most useful, multi-purpose instruments you can use in the lab. We built it with a “smart” microprocessor controller and unique true-UV-monitoring circuitry to enable you to get error-free results with greater speed, accuracy and safety than was previously possible. That’s why we also eliminated the needlessly time-consuming adjustments, unnecessarily repetitive programming and unwarranted risk of sample damage you encounter with conventional crosslinkers and other UV equipment such as light boxes and transilluminators. Our ultimate goal is to make this revolutionary new intrument your most indispensable apparatus.
Programmable, User-Friendly, “Smart” Microprocessor Controller Makes the Spectrolinker Simple and Foolproof!
Built-In “Help” Messages and LED-Function Indicators Guide You Every Step of the Way, Reminding You of:
Auto Repeat “Remembers” Previous Settings for Quick and Simple, One-Button Operation. You never have to enter the same numbers over and over. Just press “Start” to automatically repeat or to resume a user-interrupted operation. Should the door be opened during operation, the Spectrolinker alternately flashes “door” and the remaining time or energy. The “Reset” button cancels the latest settings.
Unparalleled Accuracy – Our state-of-the-art photo
Super-Fast Crosslinking – Binds Samples in Under 30 Seconds. Vacuum-oven baking would take over two hours at 80°C. The Spectrolinker is over 240 times faster!
Large UV-Blocking Window Provides Visual Confirmation — A convenient inspection window enables the user to safely observe samples and confirm that all of the UV tubes are lit.
Safeguards Your Valuable Test Results and Optimizes Binding. After investing your valuable time and effort, the last thing you want is to have your improperly bound sample disappear during the wash cycle. No need to worry about that with the Spectrolinker! Just select the desired energy level and its unique true-UV-monitoring circuitry assures that the dosage you set is always precisely delivered. Even when the tubes age or if the AC-line power fluctuates, the crosslinker’s automatic photoelectric
Dramatically Enhances Hybridization-Signal Sensitivity as compared to conventional methods. Tests were conducted at Cold Spring Harbor Laboratory (NY) to determine what effect, if any, different binding methods have on DNA retention. The autoradiographic results (on right) conclusively prove that UV irradiations with the Spectrolinker significantly improves retention over vacuum-oven baking. Three samples each of single-stranded, M13 DNA were applied in the amounts shown in the autoradiograms in GeneScreen1 nylon and 0.45µm nitrocellulose2 membranes by slot blotting. One membrane from each set of three was processed by vacuum baking for two hours at 80°C. The second and third membranes were UV irradiated by the Spectrolinker at 120mJ/cm2 (optimal) and 90mJ/cm2 dosages, respectively, and then hybridized to an RNA probe (in five-fold excess) according to Maniatis et al3. DNA retention was quantified as counts per minute (CPM) of the hybridized probe by and Ambis Radio-analytic Imaging System4.
Produces Publication-Quality Results, First Time — Every Time! The Spectrolinker greatly enhances the quality of your autoradiograms so you’ll consistently get the sharpest and clearest, publication-quality photodocumentation.
Protects the User, Too! There’s no risk of accidental UV exposure with the Spectrolinker. Its safety interlock protects you if you open the door during operation. A UV-blocking window permits easy viewing while shielding the user from harmful radiation.
Versatile — Numerous Applications include: