Advanced Capillary Electrophoresis Detectors

Advanced Capillary Electrophoresis Detectors

Groton Biosystems is dedicated to capillary electrophoresis and understands the modern laboratory's need to utilize an array of detector technologies in its day-to-day routines and methods development work. In an effort to facilitate optimum capillary electrophoresis in your laboratory, Groton Biosystems is now offering two ultraviolet absorbance detectors (fixed wavelength and variable wavelength) and a new, high sensitivity fluorescence detector (fixed wavelength) designed purposely to optimize detection at capillary scale. All of our detectors are modular and are compatible with CE, HPLC and FIA applications. These detectors can easily be mounted externally to Groton HPCE units or any other supplier's systems that are compatible with external detection systems. It is possible with our detectors to develop multiple, serial detection strategies for those difficult samples or to optimize information content of those trace quantity samples that every lab encounters from time to time. Groton HPCE instruments can even be easily interfaced to your Mass Spectrometer providing the ultimate in HPCE detection.

UV-Vis Absorbance Detectors: Fixed Wavelength, ADFW Series; Variable Single Wavelength, ADVW Series
Fluorescence Detectors: Filter Fluorescence Detector; Variable Excitation Fluorescence Detector
Contactless Conductivity Detectors

UV-Vis Absorbance Detectors

Fixed Wavelength UV-VIS Absorbance Monitor

High Sensitivity
The VUV-14 is a fixed wavelength light absorbance monitor designed to be used with ultra low volume sampling cells under flowing solvent conditions (i.e. Chromatography). The VUV series of detectors has a high-resolution analog output with a sensitivity range from 4.0 AU/V to 0.0005AU/V and feature a state-of-the-art noise level of less than +/- 0.00001 AU.

Flexible Configurations
The VUV-14D can work at any wavelength in the range from 190 to 1000 nm provided the appropriate interference filter is installed in the instrument for the desired wavelength. The VUV-14 comes standard with a low pressure Hg lamp and 254 nm filter. You can also choose a tungsten lamp to cover the wavelength ranges of 370 to 1000 nm, or a Deuterium lamp (VUV-14D) to cover wavelengths from 190 to 400 nm. Any of the lamps can be used with a 10 nm filter anywhere in their respective output ranges.

The VUV-14(D) has a broad line of sample cells available. These include cells designed for capillary "on column" monitoring, m HPLC, HPLC, nonmetallic HPLC, preparative, high-pressure preparative and square 10mm cuvette holders. What ever your application, the VUV-14 can serve as a reliable source for obtaining the results you want.

Reliable, Rugged, Low Maintenance Design
The VUV-14 is composed of a high reliability lamp, an interference filter, true double beam sample/reference light paths, a low RI high sensitivity HPLC flow cell (standard) and a robust universal power supply

Variable Single Wavelength, ADVW Series

Variable Single Wavelength UV-VIS Absorbance Monitor
The VUV-24, is one of the most versatile, reliable and sensitive visible ultraviolet absorbance monitors on the market today. The VUV-24 is particularly suited to the demands of Chromatography. The VUV-24 uses state of the art photodiode design with complimentary high-photon throughput optics. Supported by a variety of sample cell designs, this detector is capable of solving virtually every application needed in the laboratory.

The VUV-24 is capable of working from 190 to 400 nm. The working wavelength can be selected from the front panel by simple up/down arrows or through the standard serial interface.

The VUV-24 has one of the lowest noise and drift specifications in the industry. Extremely high signal to noise ratios are obtained with our standard flow cells and the sensitivity is substantially below the normal "chromatographic drift" that is routinely found in most separations.

The VUV-24 is designed for the highest linearity in absorbance possible.

The VUV-24 is designed to allow in field modifications to any of our sample cells. Available sample cells include, HPLC, m HPLC, preparative and capillary (CE and CEC).

All lamps for the VUV-24 are pre-aligned and readily accessible for in field service. The standard unit comes with a high quality long life (2000 hrs) Deuterium lamp for use from to 190 nm to 400 nm. The standard unit comes ready to be used at wavelengths as high as 940 nm with an increase in band pass in the visible region (via optional tungsten lamp).

The VUV-24 is ready to go anywhere in the world. The universal power supply has an AC input voltage of 100 to 240 Volts at 50 or 60 Hz. A rear barrier strip provides easy access to event, auto-zero and high-resolution analog Input/Output. The analog output is suitable for interface to integrators, chart recorders, or commonly used computer interface boards. Computer control and data acquisition can also be performed via the standard feature serial interface (RS-232) on the rear panel.

Fluorescence Detectors

FLD-10 Filter Fluorescence Detector

The Groton Filter Fluorescence Detector is used for chromatographic applications and is
based upon a unique system of high intensity light sources and ultra-sensitive fluorescence
detection technologies. The optical path is optimized for very high sensitivity detection of
weakly fluorescing liquids in flowing solutions. This technology has high applicability to the
chromatographic separation problems for which it is specifically designed. The FLD-10 is
designed to be the most sensitive fluorescence monitor possible for flowing or static
liquids.
Specifications:
Light Sources: Excitation from 340 nm to >1000 nm
Excitation Bandwidth: Fixed Filter Base (depending upon application) from 10 nm to
>50 nm, generally the widest bandwidth possible is used for
highest sensitivity
Emission Bandwidth: 340 nm to >1000 nm, 10 nm Bandwidth
Flow Cell: 3.0 µL Illuminated Volume, Standard 1/16" HPLC Fittings
Display: 8 Character LED Readout with Front Panel Control for
Emission Wavelength, Excitation Wavelength, Range,
Autozero, and Event
Inputs and Outputs:
o Standard RS-232 Serial Interface (bidirectional)
o High Resolution Analog Output (scaled by range select buttons)
o Event Mark (Front and Rear panels)
o Autozero (Front and Rear panels)
o Sensitivity: Signal/Noise, 50:1
o Fluorescein 3x10-10M,
o 20 µL Injection at 1.5 mL/min
o 480 nm excitation/520 nm emission
Drift: < 10 Times the Noise Level per 30 minute Period
Rise Time: 1.0 seconds
Range: 1024, 512, 256, 128, 64, 32, 16, 8, 4, 2, 1
(Attenuation Factors for Analog Output)
Dimensions: 10.5" X 7.5" X 12.5" (26 X 19 X 32 cm)
Weight: 8 LB, (3.5 kG)

Specifications subject to change without notice

FLD-20 Variable Excitation Fluorescence Detector

The Groton Variable Excitation Fluorescence Detector is used for chromatographic
applications based upon a unique system of high intensity light sources and ultra-sensitive
fluorescence detection technologies. The excitation of the sample is through a high photon
output monochromator utilizing a special high intensity ultraviolet/visible light source. The
optical path is optimized for very high sensitivity detection of weakly fluorescing liquids in
flowing solutions. This technology has applicability to the chromatographic separation
problems for which it is specifically designed. The FLD-20 is designed to be the most
sensitive fluorescence monitor possible for analyzing flowing or static liquids.
Specifications:
Light Sources: Excitation from 190 nm to >940 nm
Excitation Bandpass: Variable Monochromator base (depending upon application)
from 10 nm to >50 nm, generally bandwidth programmable
Emission Bandwidth: 190 nm to >1000 nm, application specific filters available
Standard Flow Cell: 3.0 µL Illuminated Volume, Standard 1/16" HPLC Fittings
Optional Flow Cells: 350 to 500 µm o.d. Capillary Flow Cell, 50 to 450 µm i.d.
Display: 8 Character LED Readout with Front Panel Control for Emission
Wavelength, Excitation Wavelength, Range, Autozero, and Event
Inputs and Outputs:
o Standard RS-232 Serial Interface (bi-directional)
o High Resolution Analog Output (scaled by range select buttons)
o Event Mark (Front and Rear panels)
o Autozero (Front and Rear panels)
o Sensitivity: Signal/Noise, 50:1
o Fluorescein 3X10-10M,
o 20 µL Injection at 1.5 mL/min
o 480 nm excitation/520 nm emission
o Drift: < 10 Times the Noise Level per 30 minute Period
o Rise Time: 1.0 seconds
o Range: 256, 128, 64, 32, 16, 8, 4, 2, 1
(Attenuation Factors for Analog Output)
o Dimensions: 10.5" X 7.5" X 12.5" (26 X 19 X 32 cm)
o Weight: 14 LB, (5.5 kG)
Specifications subject to change without notice.

Contactless Conductivity Detectors: Please contact our Sales Department at Sales@GiangarloScientific.com - for a complete technical information update on this exciting new line of Detectors.